|
KMID : 0361519990100020150
|
|
Korean Journal of Psychopharmacology
1999 Volume.10 No. 2 p.150 ~ p.157
|
|
|
Effects of Selective Serotonin Reuptake Inhibitors(SSRIs) on Serotonin-mediated Intracellular Ca2＋ Mobilization in Human Platelets
|
|
Han Joon-Kyu
Park Ki-Chang
Kim Hye-Kyung
Lee Joong-Woo
|
|
|
Abstract
|
|
|
Objective：The effects of treatment with selective serotonin reuptake inhibitors (SSRIs) on serotonin (5-HT)-mediated Ca2＋ mobilization were investigated in the platelets of human healthy volunteers.
Method：The serotonin (5-HT)-mediated Ca2＋ mobilization in the platelets was assessed by the fluorescence technique with fura-2/AM.
Results：SSRIs (fluoxetine, paroxetine and sertraline) themselves mobilized intracellular Ca2＋([Ca2＋]i) in a dose-dependent fashion. The increment of [Ca2＋]i might be induced partly by the release from the intracellular calcium store, but mostly induced by the calcium transport through membrane. Stimulation of platelets with 10 μM 5-HT caused a rapid and sustained increase in [Ca2＋] i levels. Resting [Ca2＋] i before 5-HT treatment was 43.37±1.25 nM. Fluoxetine inhibited the increment of [Ca2＋] i induced by 10 μM 5-HT with an IC50 value of 6.36 μM. Paroxetine augmented 5-HT-mediated increases in [Ca2＋] i, ranging from 117.76±2.79% to 316.22±18.39%, with an EC50 value of 19.68 μM. Sertraline also augmented 5-HT-mediated increases in [Ca2＋] i in a dose-dependent fashion, ranging from 106.29±3.40% to 269.29±14.96%, with an EC50 value of 11.40 μM.
Conclusions：It is likely that SSRIs increase in intracellular free calcium level directly and show the inhibiting and augmenting effects on 5-HT-mediated Ca2＋ movements. The precisemechanisms underlying the effects of 5-HT-mediated [Ca2＋] i response after treatment with SSRIs remain unclear；however, the present finding suggests the possibility that a direct, or indirect, effect to intracellular Ca2＋ signaling may be at least partly involved in the mechanism of action of SSRIs.
|
|
|
KEYWORD
|
|
|
SSRIs, Platelets, Intracellular Ca2＋, 5-HT
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|